Senior Scientist, Protein Crystallography
Posted on Apr 5, 2021 by Pfizer
We are seeking a protein crystallization specialist to join the Structural Biology group at Pfizer- Boulder Research and Development Unit. This individual will work closely with our protein crystallographer to express, purify, and crystalize a variety of challenging proteins for use in both structural and mechanistic studies.
Design and conduct protein crystallization trials, crystal optimization, and X-ray data collection.
Establish purification protocol and purify recombinant proteins from source cells.
Perform E. coli growth and recombinant protein expressions at liter-scale.
Document experimental data.
Communicate research priority, progress, and results with project teams.
Contribute to written and oral presentations related to research findings.
Provide technical and scientific guidance to junior research associates within the team.
Bachelor of Science degree with 9+ years' or Master of Science degree with 7+ years' experience in protein crystallization and protein purification for crystallization.
An expert in protein crystallization resulted in diffracting crystals and high-resolution protein crystal structures.
Independent bench-scientist capable of purifying proteins at multi-mg scale; familiar with AKTA purifier system and standard analytical techniques associated with assessing protein purification process.
Experience in recombinant protein expressions in E. coli.
Strong organizational, problem-solving, and written/oral communication skills.
Familiar with crystal handling and data collection at synchrotron facilities.
Experience with cryo-EM sample preparation protocols including negative staining and sample vitrification.
Able to lift 50lb objects with dimensions 12 inches in height and 26 inches in width.
Able to visualize microscopic objects under microscopes.
NON-STANDARD WORK SCHEDULE, ENVIRONMENT REQUIREMENTS
Able to adhere to safe work practices and perform lab works with appropriate personal protection equipment such as but not limited to safety goggles, facial mask, gloves, lab coat/protective gowning, and closed toe shoes.
Able to work at flexible hours occasionally when performing time-sensitive experiments