This Job Vacancy has Expired!

Scientist - Biology

Posted on Apr 5, 2021 by Pfizer

Pearl River, NY 10965
Immediate Start
Annual Salary

As a Scientist in the Molecular Biology team Embedded in Bacterial Vaccines and Technology, you will be at the center of our operations and you'll find that everything we do, every day, is in line with an unwavering commitment to quality. You will be part of a team that is addressing the critical task of rejuvenating our pipeline with innovative and critical vaccine programs in the years to come. The Molecular Biology team plays a key role in early antigen development for vaccines and assay development: focusing on cloning, expression, purification and preliminary antigen characterization as well as addressing research questions critical to progression of vaccine programs. With your deep knowledge in molecular biology, bacterial and viral pathogens, you will be an active team member who influences at the project team level. You will be using your scientific judgment to adapt standard methods and techniques by applying prior work experience and consulting others. Your ability to plan will help in preparing short-term work activities on projects. Your creativity in developing novel processes and new ideas will be used frequently. You will undertake mentoring activities to guide your team members.

It is your innovative scientific temperament that will help in making Pfizer ready to achieve new milestones and help patients across the globe.

How You Will Achieve It

Execute experiments to further project goals
Contribute original ideas and actively participate in planning projects
Multitasking projects and demonstrating problem solving skills in the planning and execution of objectives
Have a willingness to learn new skills and adapt to changing priorities
Interpret and summarize experimental data. Participate in the evaluation of new methods or technologies
Present experimental results at group meetings, project meetings, and department meetings, as required. Write research reports
Maintain laboratory notebook in a detailed and descriptive manner. Keep current in entries into the strains and vector databases
Contribute to achievement of goals of the team and influence at the work group/project team level
Apply technical skills and functional knowledge to projects/assignments within own work group/project team independently and proactively
Comply with all safety training and good laboratory practices


B.S./M.S. degree (NOTE: non-Ph.D.) in Molecular Biology, Microbiology, or a related discipline
Minimum of 5 years of research experience post-graduation
Minimum of 5 years of hands-on experience with plasmid cloning and protein expression in E. coli
Demonstrated knowledge of bacterial and viral pathogens including genetic knockout construction
Ability to work independently on molecular biology and protein expression projects
Proficiency in general microbiology and molecular biology techniques (Nucleic acid extraction and handling, PCR, Gibson assembly, restriction enzyme-based cloning, DNA sequencing)
Proficient in the design and construction of complex plasmid or viral vectors
Experienced user of cloning software (Vector NTI, DNAStar, Geneious)
Demonstrated skills in bacterial or viral protein expression systems (E. coli, mammalian cell handling and transfections, baculovirus/insect cell expression systems, Pichia pastoris)
Competent in protein analysis (SDS-PAGE, Western blot, quantitation by UV or colorimetric methods)
Hands-on skills in small scale protein purification approaches (eg nickel or GST affinity purification)
Ability to troubleshoot cloning, expression or purification challenges
Excellent organization and planning skills, the ability to work in a fast-paced environment independently or as part of a team, strong motivation with a dedicated work ethic
Proficiency with Microsoft Office applications and relevant scientific software, including graphing/statistical analysis tools is expected
Excellent verbal and written communication skills

Hands-on experience with ÄKTA purification systems
Familiarity with Octet Bio-Layer Interferometry and/or ELISA assays
Experience with RNA isolation, qRT-PCR and qPCR assay design and development
Experience with Illumina sequencing platforms and data analysis

Reference: 1152695444

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